A large number of strike- slip faults are distributed in the southeastern slope of the Akkule uplift in Tarim basin. The geometric and kinematic characteristics of strike- slip faults play an important role in controlling hydrocarbon migration and enrichment. S118- AT22- YUKE2, S113- AT13- YUKE3, AT20, AT18- YUKE5 faults are taken as the research object. Based on the theory of fine analysis technique of small and medium- sized strike- slip faults, detailed analysis is carried out on the layered deformation, plane segmentation, fault system activity intensity, late activity, source conditions, evolution mode and genetic mechanism of strike- slip faults. Then the petroleum geological significance is discussed based on production dynamic data. The strike- slip fault in the southeastern slope of Akkule uplift has a vertical deformation structure of deep linear fault combined with en echelon fault, and the middle- lower Ordovician strike- slip fault has obvious plane segment characteristics. The relative fault activity intensity of the southeastern slope is strong in the west and weak in the east, showing strong- weak- sub strong characteristics from north to south. The late activity of near SN- NE trending fault system is stronger than the NNE- NNW fault system in the Late Hercynian- Yanshanian period. The source condition of faults was controlled by the early rift. The source condition of main fault is better than the secondary fault. The source condition of main fault of NNE- NNW trending fault system is better than the SN- NE trending fault system, but the secondary fault is worse. The NNE- NNW trending fault system and SN- NE trending fault system were formed in the middle Caledonian, and the NEE fault was formed in the late Caledonian- early Hercynian. The strike- slip fault of the southeastern slope experienced the vertical superposition of the middle Caledonian, Late Caledonian- early Hercynian, middle- late Hercynian, and Indosinian- Yanshanian multi- stage tectonic activities. The opposite slip directions of NNE and SN main faults are due to the different fracture modes. The hydrocarbon expulsion period of source rock in the southeastern slope of Akkule uplift is in late hercynian- Himalayan age. The coupling of fault- active period and hydrocarbon expulsion period is favorable for oil- gas charging. The intensity of fault activity controls the faults source condition and reservoir size, and then controls the degree of oil and gas enrichment. The strike- slip faults with obvious late activity, intensity of fault activity and good source condition are favorable targets for further exploration.