The Zhutongjian uranium deposit, located in the northwestern part of the Xiazhuang ore field, is a breakthrough made during prospecting for uranium deposit in South China. Therefore, the discovery of uraninite- rich orebodies provides a new prospecting direction for uranium deposits in this area. The host rocks of the Zhutongjian uranium deposit are comprised of coarse- grained biotite granite and fine- grained biotite granite. In this paper, zircon LA- ICP- MS U- Pb dating, whole- rock geochemistry and Hf isotopes of the coarse- and fine- grained biotite granite were carried out to determine geochronology and petrogenesis of this deposit. Zircon LA- ICP- MS dating yields the diagenetic ages of 241.1±1.6 Ma and 233.6±1.4 Ma for coarse- and fine- grained biotite granite, respectively, indicating that the two plutons were formed in the Indasinian period. Two plutons are geochemically characterized by silica enrichment, K＞Na, strong aluminous, and low CaO/Na2O ratio. Trace elements analyses show that two plutons are enriched in LILEs (such as Rb) and HFSEs (such as Th, U, Ta, Hf), depletion in Ba, Nb, Sr, Ti, with slight positive Eu anomalies (δEu=0.212~0.269; δEu=0.155~0.258), revealing the S- type grantie. But the coarse- grained biotitic granite is of the disperser trace element patterns, indicating that it might have been affected by metasomatic alteration. In the primitive mantle normalized trace element pattern and chondrite- normalized REE diagrams, two plutons have high total REE content, distinct fractionation between LREE and HREE, right- dipping pattern, and distinct negative Eu anomalies. The Hf isotope compositions show the lower εHf(t) values of the coarse- grained biotite granite (from -10.9 to -9, with tDM2 age between 1951 Ma and 1834 Ma) and the fine- grained biotite granite (from -12.3 to -8.7, with tDM2 age between 2030 Ma and 1810 Ma), and the model ages of Paleoproterozic for the coarse- grained biotite granite (1951~1834Ma). Geochemistry and isotopic features indicate that the two plutons are strong aluminous S- type granite, and high Rb/Sr and low CaO/Na2O indicate that two plutons might be generated by partial melting of clay- rich argillaceous sedimentary rocks. Combined with the tectonic evolution of the South China, it can be concluded that the coarse- grained biotite granite formed in an extension setting during the main collision period of the Indosinian, while the fine- grained biotite granite probably formed in the transition stage of tectonic regimes from compression to extension.