The Tongling region in the MiddleLower Yangtze River belt has experienced strong magmatic activities in the Late Mesozoic, and frequent interaction of crust and mantle resulted in the formation of large amount of amphibole cumulates or megacrysts. Voluminous study suggests that amphibole cumulates or megacrysts likely resulted from the evolution of the mantle underplated magma in the lower crust magma chamber. In this study, we separated zircons from the amphibole cumulate for the first time and conducted LAICPMS UPb dating for these zircons and zircons from the host rocks. Dating analysis yielded isotopic ages of 14467±041 Ma (MSWD=025) for cumulate and 13727±041 Ma (MSWD=025) for host rock. The findings of zircons in the cumulate not only demonstrate the lower crust magmatism in the region but also provide constraint on the time of Mesozoic mantlederived magma underplating and crustmantle interaction in the Tongling region. Mineral chemistry analyses indicate that amphiboles are mainly pargasites or magnesiohastingsites, with high contents of Al2O3 (1352%~1381%) and TiO2 (263%~298%). The low Cr (178×10-6~312×10-6), low Ni (2155×10-6~2610×10-6), and low Mg number (68~69) of the amphibole imply they were not generated by primitive mantlederived magma. Comprehensive analyses in the study indicate that the magma in the deep lower crust magma chamber might have evolved into basalticandesitic magma, with amphibole being the last major crystallization phase.