The southeastern Erdos Basin presents strong phenomenon of oil and gas development. It seems that regional uranium mineralization is closely related with fade alteration. This study carried out study of organic geochemical feature of the Zhiluo Formation and its relationship with uranium mineralization in the extensively mineralized Huangling area. The gas chromatography analysis of saturated hydrocarbons shows that the peak carbon number of nalkanes in organic matter belongs to C15~C22;,with C21+ C22）/（C28+C29）from 0.72 to 26.08; ∑C21-／∑C22+of 0.88~6.39; Pr/Ph of 0.47~0.85; OEP of 0.87~1.08 and CPI of 1.04~1.22. All this data indicate that hydrocarbon source rocks are in the stage of maturation, with oil and gas organic matter mainly from lacustrine sedimentary parent material in the Triassic Yanchang Formation. The hydrocarbon generation peak of lacustrine source rocks in the Triassic Yanchang Formation in the study area is in agreement with the time of missing strata hosting uranium mineralization since the formation of Ubearing sequences. The generated oil and gas can not only provide the reduction ability for uranium mineralization，but also can make the formed uranium ore bodies avoid reconstruction by the late oxidation.