Qingshuitang Pb-Zn deposit, a medium-size deposit, is located in the northeast of Qidong County, Hunan Province. Based on field geological investigation, we carried out the sulfur and lead isotopic compositions of the ores, hydrogen and oxygen isotopic compositions of the ore-bearing quartz, as well as carbon and oxygen isotopic compositions of the ore-bearing calcites and limestone, to discuss the ore-forming material source and genesis of the Qingshuitang Pb-Zn deposit. The δ34S values of pyrite, galenite and sphalerite range from -7.41 to 2.91 ‰, and the δ34 S values of barite range from 11.49 to 12.34 ‰, indicating a deep source for magma and may be partly contaminated by the crustal substance. The 206Pb/204Pb, 207Pb/204 Pb and 208 Pb/ 204Pb ratios for ores range from of 17.810 to 18.710, 15.497 to 15.726, 37.858 to 38.834, respectively, indicating Pb was mainly derived from crust and may be mixed some mantle source. The δDSMOW and δ18O H2O values of ore-bearing quartz range from -87.4 to -79.3‰ and from 8.10 to 0.63‰, respectively, indicating that the oreforming fluid came mainly from a magmatic hydrothermal source, with meteoric water added in the late mineralization stage. The δ13CVPDB values for limestone in strata range from 0.9 to 2.6‰, the δ18OSMOW values range from 21.86 to 23.39 ‰; The δ13CVPDB and the δ18O SMOW values of orebearing calcites range from -5.3 to -4.6 ‰ and from 12.30 to 13.48‰, respectively, which are not consistent with δ13C PDB（0.9 ‰ ～ 2.6 ‰） and δ18O SMOW（21.86 ‰ ～ 23.39 ‰）of the limestone, indicating that carbon in the oreforming fluid mainly originated from deep magma. The research above shows that the ore-forming materials of the Qingshuitang deposit mainly came from the partial melting of the crust and the mixing effect was the main ore-forming mechanism.