Compared to single stable isotope tracer, the multiple stable isotopes were more effective in tracing the origin and evolution of the ore forming fluid. In this contribution, we present a combination of multiple stable isotopes, oxygen sulfur carbon included, of different minerals from Jinshandian skarn Fe deposit, Hubei province, which been proved had experienced a significant incursion of evaporites into its skarn system. Carbon and oxygen isotope values of calcite indicate extensive water rock reactions between Jinshandian pluton and limestone or dolomite, which was part of the alteration and mineralization process of the Jinshandian skarn Fe deposit. Oxygen isotope values of fluid equilibrium with minerals from different stages showed a narrow range, and exhibited a regularity variation as the decrease of temperature. It is clear that oxygen isotope values of the early skarn stage overlap within the range of magmatic water, and has markedly higher δ18O values during magnetite stage, and notable light δ18O values during post ore stages, especially in late calcite gypsum stage. These features indicating a mixing of multiple fluid sources in Jinshandian hydrothermal system, with originally dominated by magmatic water during the pre ore stage, followed by significant incursion of evaporite materials during the ore forming stage and notable influx of δ18O depleted meteoric water after ore formed. Oxygen and sulfur isotope values of anhydrite indicated incursion of heavy δ34S and δ18O enriched fluid, which was likely been the magmatic water or circulation of meteoric water with leached evaporite materials. Pyrite form magnetite stage and post ore stage (anhydrite pyrite stage) are both enriched in heavy δ34S, also suggesting the incursion of evaporite materials. The study of multiple stable isotope shows a mixing of diverse fluids in Jinshandian hydrothermal system and notable influx of evaporite materials during the ore forming stage, indicating that incursion evaporites was probably as the way of leaching evaporite materials by magmatic water or circulatory meteoric water, or a mix of both.