We measured weathering crust iron isotope composition of depth profile 1 (4m) and depth profile 2 (7m) from Huguangyan district in Zhanjiang city, Guangdong province. The result showed that the distribution range of δ57Fe in depth profile1 is 0.17～0.57‰, and is 0.20～0.68‰ in depth profile 2. Both depth profiles had heavy iron isotope composition. Iron isotope composition became heavier and heavier from bottom to top in both depth profiles, and the positive correlation between heavy iron isotope enrichment and degree of weathering happened. It implied iron isotope composition of weathering crust can be the index of the degree of weathering. On the other hand, lithosphere, hydrosphere and biosphere can be linked by weathering process because iron isotope composition of weathering crust affected by plants and leaching. Iron isotope study on weathering will greatly complement supergene geochemistry knowledge and iron isotope can be used as powerful tracer to be applied.