On the basis of shale gas accumulation theory and accumulation conditions, the controlling factors of shale gas accumulation were analyzed in this paper, which could be divided into internal and external factors. The internal control factors include shale geochemical indices, shale mineral composition and shale petrophysical indicators etc, which is the root cause of things to change and development, the source of the things development and determined the nature and development direction of things. The external control factors include reservoir depth, temperature and pressure of formation, etc, which only through internal control factors work. Things change and development mainly caused by the internal factors, where the depth, temperature, pressure and other external factors have little affect on things change and development. The internal factors and external factors of the shale gas accumulation interdependence and fight each other, thus the power and status of the contradictory position changed, which both determined the shale gas accumulation. Among them the total organic carbon type and content, total hydrocarbon content, quartz content, clay mineral content, porosity(fracture), density, pyrite content and carbonate content of the internal controlling factors are the main factors for generating shale gas reservoir. Using the multiple linear regression between two parameters, six parameters, eight parameters and gas content to obtain the formula for gas content calculation of the shale, and the gas content between the formula calculation and the measured showed linear fit well. The more parameters using, the better it fits. The paper recommended that if conditions permitting, as much as possible using a variety of parameters to predict the gas content of the shale in order to achieve better prediction results. Early in the exploration, it proposed that using the organic carbon content and the porosity which are simple and easy to get to calculate the shale gas content.