The Limahe Nickel Deposit is a typical PGE-poor Ni-Cu deposit in the Emeishan Large Igneous Province. In this paper, various kind of ore and rocks from the Limahe nickel deposit were analyzed for their Re, Os concentration and Os isotopic composition. The intrusion is heterogeneity in the initial Os isotopic composition, and does not give an unified isochron. Sulfide rich ore and their separated sulfide have higher initial radiogenic Os isotopic compositions and little difference between each other , γOs (t=260Ma) ranges from 100 to 120 and plots a Re–Os isochron corresponding to an age of 265±35 Ma, which is agreement with previous zircon SHRIMP data age of 263 ±3 Ma in the main. Peridotites and sulfide poor ore are less initial radiogenic Os. One kind of peridotite is nearly uncontaminated with values of γOs (t=260Ma) in about 5 and a less contaminated peridotite is in about 29. While the disseminated ore have initial Os isotopic composition between the sulfide rich ore and peridotites. The barren rocks and sulfide poor ore constitute a pseudo isochron with a steeper regression for their difference in initial Os isotopic composition. The isotopic variations indicate mixing of multiple components in the forming of the ore, which means that high radiogenic Os sulfides mix with less radiogenic Os magma in the staging chamber. The Cu/Pd shows the uncontaminated peridotite is primary magma origin with no sulfide segregation, by estimating, the primary magma is high in Os content in about 1 ppb, indicating it belongs to picritic magma from high degrees of partial melting. The ore sulfides have high Re/Os ratio and high initial radiogenic Os isotope compositions, the Re/Os ratio is higher than any wall-rock peridotite and estimated composition of the primary magma, all these indicate the sulfides are originated from a daughter magma that had previous sulfides segregation in the depth. Model analysis shows two stages of comtamination in lower and upper crust in proportion of about 7% each stage, in accordance with two stages of sulfide segregation.