The acritarch microfossils arediscovered from the chert nodules and bands in the Member Ⅱ of the Ediacaran Doushantuo Formation at Xiangerwan section in the western flank of Huangling anticline in the Xingshan County of Yichang, Hubei Province, South China. They are represented taxonomically by 8 species of 8 genera, including Appendisphaera grandis, Crassimembrana multitunica, Leiosphaeridia minutissima, Megasphaera inornata, Schizofusa zangwenlongii, Symphysosphaera basimembrana, Urasphaera nupta, Variomargosphaeridium gracile. Since the existence of Schizofusa zangwenlongii, it might be tentatively correlated with the second assemblage zone (Tanarium tuberosum- Schizofusa zangwenlongii) proposed by Liu and Moczydowska (2019). However, it does not contain genus Tianzhushania which is common in the Member Ⅱ of the Doushantuo Formation in the southeastern flank of Huangling anticline, there might exist environmental variations between the two flanks of the Huangling anticline.