The lack of effective paleo- geothermometers restricts the study of the thermal history of carbonate formations. Clumped isotopes, as an emerging paleo- geothermometer, is of great significance for the thermal history reconstruction of carbonate formations. The Permian is the key stratum for natural gas exploration and development in the Sichuan basin. In this paper, by testing the clumped isotopes of Permian carbonate samples in the eastern Sichuan basin and combined with vitrinite reflectance, the thermal history since Permian was reconstructed. On this basis, the thermal evolution of Permian source rocks was clarified, and the influence of the Emeishan mantle plume on the thermal regime of the eastern Sichuan basin was discussed. The clumped isotope temperature in eastern Sichuan basin is 101. 7~178. 7℃. The thermal history simulation using the exchange/diffusion model suggests that the highest paleotemperature in the study area was in the late Cretaceous. The highest paleotemperature is between 213~225℃. The paleotemperature gradient is 23~33 ℃/km. The highest paleo- heat flow in the eastern Sichuan basin was in the Permian, about 55~70 mW/m 2. The heat flow gradually decreased to the current terrestrial heat flow since the Permian. The thermal evolution analysis of source rocks shows that the thermal evolution of Permian source rocks is staged and controlled by tectonic movements. The Indosinian and Yanshan- Himalayan movements caused two stages of thermal evolution stagnation. After reaching the highest paleotemperature in the late Cretaceous, hydrocarbon generation stopped. It is in the gas generation stage nowadays. The Permian heat flow and thermal evolution of source rocks in the eastern Sichuan basin are mainly affected by crustal tension thinning, and the influence of the Emeishan mantle plume is small and limited.