Mesozoic intermediate-acid intrusive rocks are widely developed in the Tongling ore concentration area of Anhui Province, and they are closely related to the polymetallic-copper mineralization. A large number of previous studies have been issued on the genesis of these intrusive rocks, but there are still some controversies. In this paper, Tongguanshan and Dongguashan quartz diorite and Fenghuangshan and Hucun granodiorite are selected as research objects to further explore their genetic mechanism. Based on the research results of major and trace elements and Sr-Nd isotope of the whole rocks, a special effort is made to investigate the geochemical characteristics of zircon and apatite from these rocks. Integrating the previous research results and the analysis data of our job, it can be concluded that the Mesozoic intermediate-acid intrusive rocks have similar major and trace element compositions, are all of high-K calc-alkaline rock series with characteristics of arc magmatic rocks and adakitic like rocks, are also enriched in Sr-Nd isotope compositions. These geochemical characteristics combining with the petrological characteristics of mafic microgranular enclaves（MMEs）and antiband structures of amphibole and plagioclase indicate that their parental magma was generated by the mixing of mafic magma and felsic magma. Our new analytical results are as follows: the U-Pb ages of the inherited zircons are mainly in two ranges of 1.0Ga～0.8Ga and 2.4Ga～2.0Ga, the εHf(t) values of zircon grains are -20.5～-5.1 corresponding with calculated Hf two-stage model ages between 1.5Ga～2.9Ga, and the δ18O values of zircon are 5.93‰～8.85‰, the apatites have high Cl contents (average 0.3wt%) with relatively high Cl/F values (0.052～0.244), high REE contents (1714×10-6～5903×10-6) and negative δEu values (0.26～0.68). Based on the above-mentioned geochemical characteristics of major and trace elements and Sr-Nd isotopes of whole rocks, and the geochemical characteristics of singer minerals zircon and apatite obtained from this job, combined with the regional geological tectonic evolution, the different previous views on the genesis of the Mesozoic intermediate-acid intrusive rocks in the area are debated and questioned, and proposed our own point of view. It is held that the Mesozoic intermediate-acid intrusive rocks in the Tongling ore concentration area were derived from a mixture of crust-derived and mantle-derived magma, that is, the mixing of enriched lithospheric mantle derived magma and Neoproterozoic juvenile arc crustal derived magma, a small amount of ancient crustal materials from Paleo-Proterozoic to Meso-Proterozoic had also been mixed in the magma ascending process. The enriched lithospheric mantle sources magma was derived from the subducted oceanic crust or the fluid metasomatic overlying lithospheric mantle when Cathaysia oceanic slab beneath the Yangtze craton in the Neoproterozoic era, but not the Paleo-Pacific slab subducted into South China plate in the Mesozoic era. The roll-back after subduction of the Mesozoic Paleo-Pacific slab was the geodynamic mechanism that led to the ancient orogenic belt extending after thickening, the lithospheric mantle thinning and melting.