The Wanghushan ductile shear zone (WHSSZ) is located in the Yagan fault zone in Southernmost Central Asian Orogenic Belt (CAOB). In this study, we applied structural, microstructural and 40Ar/39Ar thermochronology, and U- Pb zircon geochronology to constrain the tectonic evolution of WHSSZ, and discussed its relations with subduction/collision dynamics in the Central Asian Orogenic Belt (CAOB). The WHSSZ is a nearly E- W striking ductile strike- slip shear zone, and various kinematic indicators indicate dextral shearing. The dynamic recrystallization characteristics of quartz and plagioclase indicate that the dextral ductile shearing belt is a high greenschist facies to low amphibolite facies dynamical metamorphism belt and the deformation temperature is approximately 400~500℃. LA- ICP- MS U- Pb dating were conducted on zircons from the mylonitized diorite, granite dikes, and undeformed syenogranite The weighted mean ages of diorite and syntectonic granitic vein are 331. 3±5. 7 Ma and 304. 8±2. 3 Ma. The crystallization ages of syntectonic mylonitized granite dikes and undeformed syenogranite are 308. 9±2. 0 Ma and 288. 4±1. 3 Ma, suggesting that the dextral shearing took place after 331 Ma and before 288 Ma. 40Ar- 39Ar plateau age of muscovite and biotite samples from metamorphic- deformed rocks of the WHSSZ range from 253 Ma to 237 Ma, which are consistent with the age of the Triassic magmatic rocks intrusive in the WHSSZ, and should be the record of that magmatic thermal event. We infer that the WHSSZ should be the direct record of the assembly of the middle segment of the southern CAOB during Late Carboniferous to Early Permian, and probably ended in Late Early Permian.