The Lala deposit located at the Kangdian region in the southwest margin of the Yangtze block is a representative iron oxide-copper-gold-uranium deposit in China. This deposit was produced mostly Fe-Cu mineralization;however, it was also associated with considerable U mineralization. In this study, the uranium mineral, geochemistry and U-Pb geochronology of the Lala deposit have been investigated using in-situ X-ray fluorescence spectroscopy (μ-XRF), scanning electron microscope (SEM), electron probe microanalysis (EPMA), and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) analysis to constrain the metallogenic tectonic setting and environment. The main uranium minerals found in the Lala deposit are uraninite, brannerite, and coffinite. The uraninite occurs in both Fe-Cu and U ores, whereas the brannerite and coffiniteare exclusively found in U ores. The uraninite typically occurred as cubic crystal with high U, Pb and Y contents, low Th, Ca, Si, Ti and P contents. Our U-Pb dating results suggested that there are two stages of U mineralization events in the Lala deposit. The first U mineralization, which was found in Fe-Cu ores, developed at 990±4 Ma in sync with the regional early Fe-Cu mineralization (ca. 1.05 Ga) during the continental rift setting. The second U mineralization, which evolved as late U-bearing quartz-calcite-fluorite veins in Fe-Cu ores, formed at ca. 850 Ma maintaining pace with the regional late Fe-Cu mineralization (ca. 850 Ma) under the island arc setting of active continental margin. Combined with previous research results, this study suggests that both the U mineralization formed at the high temperature hydrothermal environment, and were linked to the addition of non-magmatic fluids (e.g., meteoric water), as well as regional metamorphism, respectively.