Total organic carbon (TOC), organic carbon stable isotope (δ13C), hydrogen index (HI) and organic matter types are the effective proxies for the study of paleolimnology. This work discusses paleolacustrine characteristics (climeate, paleoproductivity and the lacustrine bottom water environments) of the upper 2nd and 3rd members of Qingshankou Formation in Songliao Basin by analyzing rock pyrolysis parameters and the data of total organic carbon. The proxies indicated that the climate changed from warm, colder, wet warm, warm but dry to dry cold periods. Sedimentary period of 1933 to 1932 m is characterized by organic lastrine facies and a high initial productivity of the lake, with a weak oxidation environment at the bottom of the lake. During other stages, the periods were dominated by organic matter with terrestrial origin; despite high content of TOC, the initial productivity of the lake is not high. TOC decreased with decreasing depth while δ13C showed a positive shift. The period of δ13C value reaching to its highest value is later than that in which TOC reached to the biggest value. During the warm stage, both TOC and HI value were higher, but δ13C value tended to be negative excursion; during the cold period, both HI and TOC value became lower, but δ13C values were positive. Therefore, these proxies can be employed to speculate paleolimnological condition and sedimentary environment.