The newly discovered graphite ore deposit in the Miaoping Mianxian County is located in the southern Qinling Mian- Lüe suture zone. The industrial type is crystalline flake graphite ore. This paper systematically analyzes the geochemical characteristics of samples and discusses the metallogenic conditions and genesis of the graphite ore deposit. The results show that the content of Al2O3 (7. 32%~15. 01%), TFeO (2. 28%~8. 68%), Na2O (0. 17%~3. 38%), K2O (1. 15%~3. 42%) and TiO2 (0. 50%~0. 80%) in biotite granulite are relatively high. The ΣREE of biotite- granulite is 106. 38×10-6~236. 37×10-6. δEu and Sr of biotite granulite show the negative anomaly, U, Cs, Pb show positive anomaly, but δCe anomaly is not obvious.The protolith of metamorphic rock in the mining area should be sedimentary rock with the characteristics of argillaceous sandstone and siltstone, which was mainly formed in the marine sedimentary environment of anoxic reduction. C isotope analysis shows that the δ13C of graphite in ore is -23. 6‰~-21. 7‰, and that of marble is -7. 1‰~3. 3‰ indicating that the graphite carbon is derived from biological organic matter, and carbon of marble from inorganic carbonate mass. Regional metamorphism helped to transform the rocks to ore zones. The genetic type of the deposit belongs to sedimentary metamorphic deposits.