The Longshan Au- Sb deposit is a typical Au- Sb ore deposit in central Hunan. Analysis and comparison of S, Pb and Sr isotopic composition of different ores, wall rocks and regional strata from the Longshan deposit have been carried out in this study. The δ 34 S values of ores vary from -3. 0‰ to 5. 1‰ with an average of 2. 3‰ and the δ 34 S values of wall rocks vary from 4. 0‰ to 5. 9‰ with an average of 5. 2‰, and the δ 34 S value of the regional strata from 9. 3 ‰ to 13. 3‰ with the average of 11. 3‰. The sulfur isotopic compositions of ores differ from the wall rocks and the regional strata, and show magmatic character. The 206 Pb/ 204 Pb, 207 Pb/ 204 Pb and 208 Pb/ 204 Pb ratios of ores are 16. 992~18. 457, 15. 392~15. 722 and 37. 586~38. 960, respectively; the 206 Pb/ 204 Pb, 207 Pb/ 204 Pb and 208 Pb/ 204 Pb ratios of wall rocks are 17. 630~17. 993, 15. 522~15. 644, 38. 981~38. 366, respectively; and the 206 Pb/ 204 Pb, 207 Pb/ 204 Pb and 208 Pb/ 204 Pb ratios of regional strata are 17. 566~18. 092, 15. 430~15. 630 and 37. 988~38. 710, respectively. The Pb isotopic compositions of ores vary greatly, and the sources are complicated, where the strata, Indosinian magma and upper mantle are all possible lead providers. The ( 87 Sr/ 86 Sr)i range of fluid inclusions in quartz is 0. 71540～0. 72309, the ( 87 Sr/ 86 Sr)i range of wall rocks is 0. 71844～0. 72153; the ( 87 Sr/ 86 Sr)i range of regional strata is 0. 71792～0. 71939. The initial Sr values of ore rocks, wall rocks and regional strata are all relatively high, suggesting that they might mainly have come from the crust, with some contribution of Sr from ore- bearing strata and the Indosinian magma. The ore- forming materials in the Longshan deposits show characteristics of crust- mantle mixture, in which the ore- forming sulfur originated from magma while the other ore- forming material are partially from the regional strata of the Jiangkou Formation, and partially from the Indosinian magma.