Determination of the origin of methane- rich fluids is an important issue in study of cold seep. Sr isotope as a tracer can reflect fluid sources, fluid mixing and fluid- rock reactions. In recent years, Sr isotope of cold seep carbonates has recieved much worldwide attention. This paper summarized the research progress of Sr isotopes of modern and ancient cold seep carbonates, including the testing methods of Sr isotope and preprocessing method comparison of sample dissolution using different acid solution, and finally pointed out the necessity of establishing the standardized processing methods. The Sr isotopic compositions of modern and ancient seep carbonatic rock vary significantly with different carbonatic minerals. The study of Sr isotope tracer of cold seep carbonates mainly focused on the following aspects: qualitative examination of modern and ancient seep fluid sources, quantitative model calculation of mixed fluid sources, and 87Sr/86Sr isotopic dating of fossil methane seep carbonates. Although a series of progress was achieved, the accuracy of fluid mixing ratios using quantitive calculation of end members needs to be improved. Meanwhile, the uncertainties still occur in Sr isotopic dating for fossil seep carbonates, and thus its application conditions need further to study.