The Matou molybdenumcopper deposit is a mediumsized polymetallic deposit which was newly ascertained in south Anhui of the Middle and Lower Reaches of the Yangtze River, and the mineralization was constrained by the transformation mechanisms which were changed from the EWtrending PaleoTethys techonic system in to the ancient NEtrending. Pacific tectonic system in the characteristics of fluid inclusion and stable isotope were studied to determine the genesis and to construct the metallogenic pattern for the deposit. The results show that the homogenization temperature range for fluid inclusions in quartz from 91 ℃ to 398 ℃, the salinity range from 088％to 374％ and the density range from 018g/cm3 to 110g/cm3, those indicated that the oreforming fluid was medium temperature, medium salinity and lower density fluid, the potassium feldspar alteration, quartzsericitization and propylitic alteration stages were experienced in the oreforming fluid evaluation, and the mass mineralization were occurred in quartzsericitization stage. Pb isotopic composition in ore were similar to wall rock, the indistinguishable Pb isotopic composition with ore and wall rock in 208Pb/204Pb206Pb/204Pb figure indicated that oreforming materials are multisources, and more mineralizing matters were supplied by granodioriteporphyry. The δ34S values of pyrite show a total range from -100‰ to +700‰ and has a tower type distribution, the δ34S values of molybdenite were higher than pyrite, showing that the sulfur isotopic composition reached a equilibrium states in minerals, which were mainly from deep mantle and contaminated by crustal sulfur. The oxygen isotopic composition (δ18O values) of free quartz and orebearing quartz vein show a range from 980‰ to 130‰, both of them were formed in the same hydrothermalism, the δ18Owater values in fluid show a range from -121‰ to +199‰ are similar to the composition in atmospheric precipitation, those inferred that the original orebearing hydrothermal were mixed by atmospheric precipitation in different degree in metallogenic process. The Matou deposit is a subvolcanic hydrothermal deposit which has a characteristics of wall rock alteration for porphyry deposit, and belongs to a porphyry metallongic system. Granodioriteporphyry is the main mother rock which supplied the main oreforming materials for mineralization in the mining area, and siltstone supplied certain material conditions as well, but moyite veins unrelated to the mineralization.