Sulfur stable isotope is widely used to trace the water source, material composition and water-rock interaction of the underground water and surface. The surface water (Jialing river), Sand pit water, Qingmuguan Karst underground water(Jiangjia spring), Shallow geothermal water in karst area (The north hot spring and Qingmuguan hot spring) in Wentanggorge anticline in the eastern Sichuan parallel Ridge and Valley were studied, and the chemical features of Jialing river and Jiangjia spring are showing the characteristics of the carbonate chemical weathering, however, the chemical composition of the shallow geothermal water is giving an account of the sulfate chemical weathering. It was found that sulfur isotopic tracingindicate different environmental significance in different water by the study of the SO42-与δ34S-SO42-in these water. The concentration of SO42-and the value of δ34S-SO42-inJialing river indicate that the source of the Jialing river is the surface water in study area and the rain water, whichinSand pit water of study area also indicated the water source that make up by the older pit water, rain water and surface water.But the concentration of SO42- and the value of δ34S-SO42-in Qingmuguan underground water showing that the Qingmuguan underground water is mainly effected bystrata，rainfall，agriculture and dmoestic wastewater. However, the concentration of SO42- and the tracer ofδ34S-SO42-appeared that the water-stone reaction of this aquifer reservoir is mainly the gypsumdissolution.