Tongshanling copper-polymetallic ore field is a typical W-Sn-Pb-Zu-Cu polymetallic deposit in south Hunan Province, China. This study has carried on S-Pb-C isotopic anlaysis on various types of rocks and minerals. The δ34S value varies from -1.9‰ to 5.7‰ with the average value of 2.6‰, implying the main source of sulfur comes from the magma with homogeneous S isotopic composition. The equilibrium temperature of S isotopic shows the mineralization temperature is 134~339℃. The 06Pb/204Pb, 207Pb/204Pb and 208Pb/204Pb ratio values of ore rocks are 18.256～18.856, 15.726～15.877 and 38.352～39.430, respectively; The 06Pb/204Pb, 207Pb/204Pb and 208Pb/204Pb ratio values of wall rocks are 18.617～18.805, 15.721～15.786 and 38.923～39.073 respectively. The two types of rock with consistent Pb composition of upper crust Pb, indicating that they originate from magma fractionation with an origin of ancient basement rock. The δ13CPDB value of different types of rock and mineral calcite ranges from -9.88‰ to 1.32‰; the δ18OSMOW value ranges from 11.67‰ to 17.68‰. The δ18OSMOW and δ13CPDB value of calcite mineral increase gradually from the skarn rock to further wall rock, so the carbon in ore-forming minerals may generally come from magma, with some carbonate straitgraphic carbon involved later on. The ore-forming material in Tongshanling ore fields mainly comes from magma, while the strata offer limited supply to the mineralization with small amounts of ore-forming material.