Organic-inorganic interaction is ubiquitous in the process of formation, evolution, concentration and accumulation of multi-energy resources of oil, gas, coal and uranium. Uranium also alter the process of hydrocarbon generation and evolution of hydrocarbon source rocks while that organic matter accelerate concentration and mineralization of uranium by the strong adsorption and reduction environment from the organic matter. Hydrocarbon- generating simulation experiment is proceeded using hydrocarbon source rock containing kerogen type Ⅱ with uranium (UO2CO3 solution) added to study the effects of uranium on the hydrocarbon generation of hydrocarbon source rocks by comparative study of relevant parameters of experiment products. Experiment results show that uranium can facilitate unsaturated hydrocarbons changing to saturated hydrocarbons, promoting the breaking of long-chain hydrocarbons, the low molecular weight hydrocarbons generating, as a result the content of CH4 increased, the degree of dry gas increased. Uranium can promote the maturity of organic matters, reduce the hydrocarbon generation threshold values and advance the generation of the hydrocarbons of immature hydrocarbon source rocks at low temperature, while delay the over mature of organic matter that benefit to the preservation of hydrocarbons at high temperature. It means that uranium in hydrocarbon source rocks is maybe one of the inorganic accelerating factors of the hydrocarbon generation.