The Tushibulake Formation with typical section located in the Quruqtagh area is a clastic sedimentary succession consisting of greyish sandstones, siltstones, tuffaceous sandstones and siltstones, and minor limestones. Detrital zircon U-Pb ages from 3 sandstone samples were measured by the LA-MC-ICP-MS method. The samples record 183 age data that provide constraints on the stratigraphic age of the Tushibulake Formation and regional tectonic evolution. 14 credible U-Pb age data obtained indicate Tushibulake Formation deposited in Middle to late Silurian. All zircon U-Pb dates indicate that sediments of Tushibulake Formation are mainly from 422~537Ma、559~999Ma、1018~1574Ma and 1604~2498Ma source rocks. 394~537Ma detrital zircons and CL images indicate that there occurred a large magmatism though magmatic rock records in outcrop are few. During Neoproterozoic, zircons of magmatic origin show that supercontinent Rodinia assembly and break-up resulted in igneous rocks. Based on CL images and zircon U-Pb ages, there occurred a few metamorphic events, part of which were related with supercontinent Columbia.