The Ediacaran is characterized by diversification of many groups in microfossils record, including filamentous cyanobacteria, multicellular algae and acritarchs. A new microfossils assemblage from the Ediacaran Doushantuo Formation at Zhangcunping, northern Yichang, China, includes filamentous cyanobacteria (Oscillatoriopsis, Polytrichoides， Salome，Siphonophycus), multicellular algae (Sarcinophycus, Wengania) and acritarchs (Appendisphaera, Ericiasphaera, Knollisphaeridium, Leiosphaena, Meghystrichosphaeridium and Tianzhushania) . Acritrachs are found at the first time in the chert nodules in this region. The new microfossil assemblage is similar to that from the member II of the Doushantuo Formation in the East Yangtze Gorges area and the upper phosphorites in Weng’an, central Guizhou.