Hukeng tungsten, located in Wugongshan metallogenic belt in central part of Jiangxi Province, is one large scale quartz vein type wolframite deposit. For the purpose of studying the chronology of the deposit, the authors purified molybdenites associated with wolframite from W-bearing quartz veins in the Hukeng tungsten deposit. Re-Os dating of these molybdenite minerals yielded isochron age of 150.2±2.2Ma and weighted average model age of 149.82±0.92Ma. These results indicate the Hukeng tungsten deposit formed in about 150Ma and was the product during the explosive period of large scale petrogenesis and metallogenesis in Mesozoic in South China. The nolybdenites from the Hukeng tungsten deposit has a very low content of Re, showing a crustal source for ore-forming materials. The deposit formed under the Yanshanian lithospheric extensional and thinning environment..