Detailed research of typical paleo-reservoir and remnant oil/gas reservoir in the depression of southern Guizhou bears great significance in exploration of sea-facies carbonate layers.This paper analyzed geochemistry and oil source of Cambrian-Permian oil seepage and solid bitumen in the region of southeast Guizhou by using carbon isotope,saturated hydrocarbon chromatography,saturated hydrocarbon GC-MS,and aromatic hydrocarbon GC-MS.The results indicated that No.47 oil well at Huzhuang and oil seepage hosted in the Silurian sandstone at Luomian as well as the slid bitumen of Ordivician O1h member in the Majiang area are of the same oil source,mainly originating from high-quality hydrocarbon source rocks of the Lower-Cambrian Niutitang Formation,while the oil seepage of P1m in Majiang is obviously different from that of No.47 oil well at Huzhuang,it comes mainly from the high-quality hydrocarbon source rocks of the Lower-Permian Qixia Formation.