Totally 50 samples of crude oils with different genetic types were investigated using TSF (Total Scanning Fluorescence) techniques to unravel their 3D fluorescence features and controlling factors. The oils analyzed include immature to highly matured oils, lacustrine oils with source rocks developed in fresh water and brackish-hypsaline environment, oils from paludal facies, high waxy oils with relatively low sulfur content and low waxy oils with high sulfur content, light oil and heavy oils. The investigation shows that the oils are different in both fluorescent fingerprints and TSF parameters. It was observed that the TSF intensity of the oils increases with aromatic concentration, and the oils from paludal facies have higher TSF intensity than that of the lacustrine oils. It was also observed that there is an increasing trend of the oil TSF intensity with increasing maturity, and a shortening trend of the wave length of the prominent peak, indicating a decreasing trend of the total aromatic concentration as well as those aromatics with relatively higher molecular weighty. The results show that the fluorescent fingerprints of the heavy oils are different from that of the normal oils obviously, which are characterized by much wider excitation and emission wavelength without apparent main peak. We suggest that maturity, kerogen type and secondary alteration all have a strong influence on the 3D fluorescence of the oils.