Totally 40 normal oils, 15 fluid inclusion oils and 39 reservoir rock extracts from the Dongying Depression were investigated using TSF (Total Scanning Fluorescence) and GQF-E (Quantitative Grain Fluorescence on Extract) techniques combined with biomarker analysis to unravel their 3-D fluorescence features characteristically and quantitatively. It was observed that both the normal oils and the inclusion oils show generally similar TSF fingerprints with only one spectral peak, suggesting close genetic relationship of the oils. It was also observed that the peak-wide and maximum intensity of the TSF increase with decreasing oil maturity. The results show good positive relationship between the TSF indices i.e., TSF intensity, TSF R1(=Em_ 360nm /Em_ 320nm ) as well as TSF R2(=Em_ 360nm /Em_ 320nm ) and the biomarker maturity parameters, i.e., C_ 29 sterane 20S/(S+R), Ts/(Tm+Ts), which suggest that the former can be used as potential indicators of thermal maturity. According to the TSF thermal indices, we observed the oils in the Central Uplift of the Dongying Depression that exhibit a decreasing thermal maturity trend from the southwest to the northeast, indicating variation of the oil sources and primary oil charging direction. QGF-E analysis of the tight sand or siltstone extracts from Niu876 and Wang 550 wells in the Niuzhuang Sag showed that most of the samples bear relatively strong TSF intensity, indicating existence of relatively high abundance of hydrocarbons and therefore possibly well development of subtle oil-migrating pathways such as thin sand/siltstone and microfissure in the Niuzhuang Sag, which is significant for the formation of the subtle oil pools in the Niuzhuang Sag by connecting lithologic traps and deep source rocks. This study shows good prospect of the TSF and QGF-E techniques in discriminating oil group, evaluating maturity and identifying oil migration pathway as well as paleo-oil layer.