The Ludong area in the Junggar Basin can be divided vertically into two petroleum migration-accumulation fluid dynamic systems: the abnormal deep high-pressure system and the normal middle-shallow low-pressure system. In the former case, homogenization temperatures of fluid inclusions can be effectively used to determine the time of hydrocarbon accumulation and the filling history. However, it is not applicable to the latter case. This is mainly because of the different fluid dynamic environments of the two systems, which influence fluid phases when the inclusions are captured. In the abnormal high-pressure fluid dynamic environment, the inclusions are easily formed in a homogenization phase, while in the normal low-pressure system it is possible that the inclusions are captured in multiple phases. Therefore, the homogenization temperatures of fluid inclusions cannot be applied to determining the time of hydrocarbon accumulation and the filling history. It is emphasized that the corresponding fluid dynamic conditions should be considered when the homogenization temperatures of fluid inclusions are used to determin the time of petroleum accumulation.